Interaction between avian influenza subtype H9N2 and Newcastle disease virus vaccine strain (LaSota) in chickens

Isolation and Identification of Avian Influenza Virus H9N2 Subtype

Monitoring virus harvesting time in embryonated chicken eggs inoculated with avian influenza H9N2 vaccine strain

Knowledge of virus and replication kinetic is one of the most important issues in the vaccine production. The present study aimed to evaluate the best harvesting time of H9N2 avian influenza virus (AIV) vaccine strain inoculated in specific pathogen free (SPF) embryonated chicken eggs (ECE)s. For this purpose, 10-5 dilution of AIV (A/Chicken/Iran/99/H9N2) was inoculated into 336, 11-day old ECE...

Optimizing the process of inactivating influenza virus subtype H9N2 by formalin in the production of killed avian influenza vaccine

Avian influenza is one of the most important diseases in avian industry, which also threats human population. Thus, vaccination is necessary for controlling this viral disease. In this study, killed vaccine of avian influenza subtype H9N2 and formalin solution (for virus inactivation) were used. It is necessary to study the effect of different factors such as formalin concentration, as well as ...

Isolation of H9N2 Subtype of Avian Influenza Viruses during an Outbreak in Chickens in Iran

Avian influenza is an important disease of poultry with the potential to cause major epidemics resulting in significant economic losses. The presence of avian influenza viruses (AIV) in chickens in Iran has not been previously reported. An avian influenza outbreak in broiler, layer and breeder farms occurred during a very hot summer in July 1998. Three AIV isolates designated as 101, 102 and 10...

Newcastle disease virus expressing H5 hemagglutinin gene protects chickens against Newcastle disease and avian influenza.

Newcastle disease virus (NDV)-expressing avian influenza virus (AIV) hemagglutinin (HA) of subtype H5 was constructed by reverse genetics. A cloned full-length copy of the genome of the lentogenic NDV strain Clone 30 was used for insertion of the ORF encoding the HA of the highly pathogenic AIV isolate A/chicken/Italy/8/98 (H5N2) in the intergenic region between the NDV fusion and hemagglutinin...